Tutorial: NGLess

tl;dr: Learn how to use NGLess from the original developers. In particular, we will see how to run NGLess on a set of of human gut samples.

Prerequisites

What you should know/be able to do before the tutorial:

• You should know what a FastQ file is
• You should be able to run programmes on the command line
• You should know some basic scripting (e.g., run a Python or R script)

You will also be sent some information a few days before the tutorial with both the signup links & and some requests to prepare for the event. You should expect to spend some time installing the software and downloading the tutorial data.

Location & Schedule

Date: 25th June or 5th July, 2021 (these will be the same tutorial, just two separate sessions due to demand)

Location: Zoom & Discord (links will be sent to registered participants)

Calendar link

All times are UTC:

• 12h00 (Noon) – 12h27: Luis Pedro Coelho: General background on NGLess & metagenomics processing approach
• 12h27 – 12h30: 3 minute break
• 12h30 – 13h27: hands on tutorial
• 13h30 – 13h57: Open Q&A

Background information

• NGLess webpage: https://ngless.embl.de/
• NGLess manuscript: Coelho et al., 2019
• Podcast episode (from the excellent Bioinformatics Chat podcast) on NGLess: https://bioinformatics.chat/ngless

You can also join the NGLess mailing list for user questions and support requests (although bugs should be reported as Github issues).

To register

Deadline: June 22 2021 any timezone (first-come, first-served).

https://forms.gle/7jUkvZyqtNsCuz4o8